The development of biologics, including protein and gene agents, represents a major advancement towards better therapeutics with high potency and specificity. Their broader adoption, however, has been limited by factors such as undesired biodistribution, low stability, and immunogenicity. The Wen lab focuses on developing advanced biologics through nano-encapsulation and molecule-architecture design that mitigate such limitations, leading to various therapeutics for viral infection, central nervous system diseases (e.g., brain tumor, systemic tumor with brain metastases, and spinal cord injury), and cancers via immune modulation and other pathways.
Publications
- Zhang X, Son R, Lin YJ, Gill A, Chen S, Qi T, Choi D, Wen J, Lu Y, Lin NYC, Chiou PY. Rapid prototyping of functional acoustic devices using laser manufacturing.. Lab on a chip, 2022.
- Wang L, Cao Z, Wang Z, Guo J, Wen J. Reactive oxygen species associated immunoregulation post influenza virus infection.. Frontiers in immunology, 2022.
- Agbosu EE, Ledger S, Kelleher AD, Wen J, Ahlenstiel CL. Targeted Nanocarrier Delivery of RNA Therapeutics to Control HIV Infection.. Pharmaceutics, 2022.
- Castillo R, Wu D, Cao Z, Yan R, Fajardo K, Ren J, Lu Y, Wen J. Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes.. Methods in molecular biology (Clifton, N.J.), 2022.
- Yan R, Ren J, Wen J, Cao Z, Wu D, Qin M, Xu D, Castillo R, Li F, Wang F, Gan Z, Liu C, Wei P, Lu Y. Enzyme Therapeutic for Ischemia and Reperfusion Injury in Organ Transplantation.. Advanced materials (Deerfield Beach, Fla.), 2021.
- Wen J, Cheever T, Wang L, Wu D, Reed J, Mascola J, Chen X, Liu C, Pegu A, Sacha JB, Lu Y, Haigwood NL, Chen ISY. Improved delivery of broadly neutralizing antibodies by nanocapsules suppresses SHIV infection in the CNS of infant rhesus macaques.. PLoS pathogens, 2021.
- Wen J, Wang L, Ren J, Kranz E, Chen S, Wu D, Kanazawa T, Chen I, Lu Y, Kamata M. Nanoencapsulated rituximab mediates superior cellular immunity against metastatic B-cell lymphoma in a complement competent humanized mouse model.. Journal for immunotherapy of cancer, 2021.
- Qin M, Cao Z, Wen J, Yu Q, Liu C, Wang F, Zhang J, Yang F, Li Y, Fishbein G, Yan S, Xu B, Hou Y, Ning Z, Nie K, Jiang N, Liu Z, Wu J, Yu Y, Li H, Zheng H, Li J, Jin W, Pang S, Wang S, Chen J, Gan Z, He Z, Lu Y. An Antioxidant Enzyme Therapeutic for COVID-19.. Advanced materials (Deerfield Beach, Fla.), 2020.
- Suryawanshi GW, Khamaikawin W, Wen J, Shimizu S, Arokium H, Xie Y, Wang E, Kim S, Choi H, Zhang C, Yu H, Presson AP, Kim N, An DS, Chen ISY, Kim S. The clonal repopulation of HSPC gene modified with anti-HIV-1 RNAi is not affected by preexisting HIV-1 infection.. Science advances, 2020.
- Qin M, Wang L, Wu D, Williams CK, Xu D, Kranz E, Guo Q, Guan J, Vinters HV, Lee Y, Xie Y, Luo Y, Sun G, Sun X, He Z, Lu Y, Kamata M, Wen J, Chen ISY. Enhanced Delivery of Rituximab Into Brain and Lymph Nodes Using Timed-Release Nanocapsules in Non-Human Primates.. Frontiers in immunology, 2020.
- Wu D, Yang Y, Xu P, Xu D, Liu Y, Castillo R, Yan R, Ren J, Zhou G, Liu C, Qin M, Du J, Hou L, Chen I, Kang C, Jin L, Wen J, Chen W, Lu Y. Real-Time Quantification of Cell Internalization Kinetics by Functionalized Bioluminescent Nanoprobes.. Advanced materials (Deerfield Beach, Fla.), 2019.
- Wen J, Wu D, Qin M, Liu C, Wang L, Xu D, Vinters HV, Liu Y, Kranz E, Guan X, Sun G, Sun X, Lee Y, Martinez-Maza O, Widney D, Lu Y, Chen ISY, Kamata M. Sustained delivery and molecular targeting of a therapeutic monoclonal antibody to metastases in the central nervous system of mice.. Nature biomedical engineering, 2019.
- Xu D, Wu D, Qin M, Nih LR, Liu C, Cao Z, Ren J, Chen X, He Z, Yu W, Guan J, Duan S, Liu F, Liu X, Li J, Harley D, Xu B, Hou L, Chen ISY, Wen J, Chen W, Pourtaheri S, Lu Y. Efficient Delivery of Nerve Growth Factors to the Central Nervous System for Neural Regeneration.. Advanced materials (Deerfield Beach, Fla.), 2019.
- Wu D, Qin M, Xu D, Wang L, Liu C, Ren J, Zhou G, Chen C, Yang F, Li Y, Zhao Y, Huang R, Pourtaheri S, Kang C, Kamata M, Chen ISY, He Z, Wen J, Chen W, Lu Y. A Bioinspired Platform for Effective Delivery of Protein Therapeutics to the Central Nervous System.. Advanced materials (Deerfield Beach, Fla.), 2019.
- Han L, Liu C, Qi H, Zhou J, Wen J, Wu D, Xu D, Qin M, Ren J, Wang Q, Long L, Liu Y, Chen I, Yuan X, Lu Y, Kang C. Systemic Delivery of Monoclonal Antibodies to the Central Nervous System for Brain Tumor Therapy.. Advanced materials (Deerfield Beach, Fla.), 2019.
- Khamaikawin W, Shimizu S, Kamata M, Cortado R, Jung Y, Lam J, Wen J, Kim P, Xie Y, Kim S, Arokium H, Presson AP, Chen ISY, An DS. Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously Infected with HIV-1.. Molecular therapy. Methods & clinical development, 2017.
- Chan J, Kim PY, Kranz E, Nagaoka Y, Lee Y, Wen J, Elsaesser HJ, Qin M, Brooks DG, Ringpis GE, Chen ISY, Kamata M. Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming.. AIDS research and human retroviruses, 2017.
- Li J, Zhang L, Liu Y, Wen J, Wu D, Xu D, Segura T, Jin J, Lu Y, Wang H. An intracellular protein delivery platform based on glutathione-responsive protein nanocapsules.. Chemical communications (Cambridge, England), 2016.
- Tian H, Du J, Wen J, Liu Y, Montgomery SR, Scott TP, Aghdasi B, Xiong C, Suzuki A, Hayashi T, Ruangchainikom M, Phan K, Weintraub G, Raed A, Murray SS, Daubs MD, Yang X, Yuan XB, Wang JC, Lu Y. Growth-Factor Nanocapsules That Enable Tunable Controlled Release for Bone Regeneration.. ACS nano, 2016.
- Wen J, Yan M, Liu Y, Li J, Xie Y, Lu Y, Kamata M, Chen IS. Specific Elimination of Latently HIV-1 Infected Cells Using HIV-1 Protease-Sensitive Toxin Nanocapsules.. PloS one, 2016.
- Yan M, Wen J, Liang M, Lu Y, Kamata M, Chen IS. Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs.. PloS one, 2015.
- Liu C, Wen J, Meng Y, Zhang K, Zhu J, Ren Y, Qian X, Yuan X, Lu Y, Kang C. Efficient delivery of therapeutic miRNA nanocapsules for tumor suppression.. Advanced materials (Deerfield Beach, Fla.), 2014.
- Yan M, Liang M, Wen J, Liu Y, Lu Y, Chen IS. Single siRNA nanocapsules for enhanced RNAi delivery.. Journal of the American Chemical Society, 2012.
- Wen J, Anderson SM, Du J, Yan M, Wang J, Shen M, Lu Y, Segura T. Controlled protein delivery based on enzyme-responsive nanocapsules.. Advanced materials (Deerfield Beach, Fla.), 2011.
- Chen Z, Augustyn V, Wen J, Zhang Y, Shen M, Dunn B, Lu Y. High-performance supercapacitors based on intertwined CNT/V2O5 nanowire nanocomposites.. Advanced materials (Deerfield Beach, Fla.), 2011.
- Bian Z, Zhu J, Wen J, Cao F, Huo Y, Qian X, Cao Y, Shen M, Li H, Lu Y. Single-crystal-like titania mesocages.. Angewandte Chemie (International ed. in English), 2010.