Kenneth Dorshkind

Kenneth A Dorshkind

Professor, Pathology and Laboratory Medicine, University of California Los Angeles

(310) 206-9535

Laboratory Address:
Factor 11-640

Lab Number:
310 206-9301

Work Address:
A2-179 CHS
CAMPUS - 173216
CA Factor
Los Angeles, CA 90095


Member, Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, JCCC Cancer and Stem Cell Biology Program Area, Molecular, Cellular & Integrative Physiology GPB Home Area

Research Interests

The overall goal of the laboratory is to study B cell development during fetal and adult life. The first B lineage cells emerge in the embryo, and recent studies have demonstrated that these arise from a B lineage progenitor whose developmental potential is restricted to the production of B-1 B cells. B-1 B cells are a minor population of B lymphocytes that are involved in innate immune responses. B cell progenitors that derive from hematopoietic stem cells, and which are destined to produce B-2 B cells, arise subsequently during embryogenesis. B-2 B cells comprise the majority of B cells in the peripheral lymphoid organs and take part in adaptive immune responses. The production of B-2 B cells occurs in the bone marrow throughout life. One aim of the laboratory is to characterize the fetal wave of B cell development in which B-1 B cell progenitors are generated. Emerging data indicate that the transcriptional and microenvironmental regulation of B-1 B cell development is distinct from B-2 B cell differentiation, and a major goal is to compare and contrast these two waves of B lymphopoiesis. Additional work in the laboratory has demonstrated that B-2 B cell development in the bone marrow is reduced with age, and ongoing studies are aimed at defining the microenvironmental and cell intrinsic events that underlie this decline.


B and T Cell Development The development of B and T lymphocytes occurs in the bone marrow and thymus, respectively, in association with a supporting framework of stromal cells. One aim of our work is to characterize immature lymphoid progeny derived from pluripotential hematopoietic stem cells. A current focus of the laboratory is on the characterization of a B/macrophage progenitor that has recently been identified in adult bone marrow. The origin of these cells and the contribution of their progeny to the adult immune system is being assessed. Another major aim of the laboratory is to define the basis for thymic involution during aging and to develop protocols for rejuvenation of that organ. Multiple in vitro and in vivo systems have been developed to facilitate these studies. Students who enter the laboratory receive state of the art training in such techniques as cell culture, flow cytometry, and bone marrow transplantation. This background provides then the skills necessary to address issues in Hematopoiesis and Developmental Immunology.