Ambre Marguerite Solange Bertholet
Assistant Professor, Physiology, University of California Los Angeles
Laboratory Address:
10833 Le Conte Avenue
10833 Le Conte Avenue, Los Angeles
Los Angeles, CA 90095
Office Address:
10833 Le Conte Avenue, Los Angeles
Los Angeles, CA 90095
Affiliations
Research Interests
Mitochondria control cell metabolism by converting nutrients into an electrochemical gradient of protons (H+) across the inner mitochondrial membrane (IMM) to generate ATP, the currency of the cell, and heat (a process called mitochondrial thermogenesis). This energy conversion depends on H+ leaks across the IMM. A fine balance of H+ distribution between the two energy production forms, ATP and heat, defines the metabolic homeostasis of the cell. How mitochondria distribute energy between ATP and heat is certainly the most fundamental question in the field of bioenergetics, although it is still largely unknown. Our lab will explore the mechanisms of bioenergetics using the novel mitochondrial patch-clamp assay combined with modern cellular and molecular techniques with the goal of providing a fundamental understanding of how energy production and basal metabolism control physiology.
Biography:
Dr. Bertholet was trained in Toulouse, France at the Université Paul Sabatier. She earned her B.S. in Cell Biology and Physiology, M.S. in Gene Cells and Development, and her Ph.D. in Biology, Health and Biotechnologies. Subsequently, she did her postdoctoral training with Dr. Yuiry Kirichok at UC San Francisco and is currently an Assistant Research Physiologist.
Publications
- Chang CF, Gunawan AL, Liparulo I, Zushin PH, Bertholet AM, Kirichok Y, Stahl A. CoQ Regulates Brown Adipose Tissue Respiration and Uncoupling Protein 1 Expression.. Antioxidants (Basel, Switzerland), 2022.
- Bertholet AM, Natale AM, Bisignano P, Suzuki J, Fedorenko A, Hamilton J, Brustovetsky T, Kazak L, Garrity R, Chouchani ET, Brustovetsky N, Grabe M, Kirichok Y. Mitochondrial uncouplers induce proton leak by activating AAC and UCP1.. Nature, 2022.
- Bertholet AM, Kirichok Y. Mitochondrial H+ Leak and Thermogenesis.. Annual review of physiology, 2021.
- Bertholet AM. The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria.. Journal of visualized experiments : JoVE, 2021.
- Allu PKR, Paulo E, Bertholet AM, Situ G, Lee SH, Wu Y, Gleason CE, Saha B, Chawla A, Wang B, Pearce D. Role of mTORC2 in biphasic regulation of brown fat metabolism in response to mild and severe cold.. The Journal of biological chemistry, 2021.
- Bertholet AM, Kirichok Y. Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat.. Frontiers in physiology, 2020.
- Bertholet AM, Chouchani ET, Kazak L, Angelin A, Fedorenko A, Long JZ, Vidoni S, Garrity R, Cho J, Terada N, Wallace DC, Spiegelman BM, Kirichok Y. H+ transport is an integral function of the mitochondrial ADP/ATP carrier.. Nature, 2019.
- Bertholet AM, Kirichok Y. The Mechanism FA-Dependent H+ Transport by UCP1.. Handbook of experimental pharmacology, 2019.
- Odegaard JI, Lee MW, Sogawa Y, Bertholet AM, Locksley RM, Weinberg DE, Kirichok Y, Deo RC, Chawla A. Perinatal Licensing of Thermogenesis by IL-33 and ST2.. Cell, 2017.
- Bertholet AM, Kazak L, Chouchani ET, Bogaczynska MG, Paranjpe I, Wainwright GL, Bétourné A, Kajimura S, Spiegelman BM, Kirichok Y. Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling.. Cell metabolism, 2017.
- Bertholet AM, Kirichok Y. UCP1: A transporter for H+ and fatty acid anions.. Biochimie, 2016.
- Odegaard JI, Lee MW, Sogawa Y, Bertholet AM, Locksley RM, Weinberg DE, Kirichok Y, Deo RC, Chawla A. Perinatal Licensing of Thermogenesis by IL-33 and ST2.. Cell, 2016.
- Millet AM, Bertholet AM, Daloyau M, Reynier P, Galinier A, Devin A, Wissinguer B, Belenguer P, Davezac N. Loss of functional OPA1 unbalances redox state: implications in dominant optic atrophy pathogenesis.. Annals of clinical and translational neurology, 2016.
- Bertholet AM, Millet AM, Guillermin O, Daloyau M, Davezac N, Miquel MC, Belenguer P. OPA1 loss of function affects in vitro neuronal maturation.. Brain : a journal of neurology, 2013.
- Landes T, Leroy I, Bertholet A, Diot A, Khosrobakhsh F, Daloyau M, Davezac N, Miquel MC, Courilleau D, Guillou E, Olichon A, Lenaers G, Arnauné-Pelloquin L, Emorine LJ, Belenguer P. OPA1 (dys)functions.. Seminars in cell & developmental biology, 2010.
- Betourne A, Bertholet AM, Labroue E, Halley H, Sun HS, Lorsignol A, Feng ZP, French RJ, Penicaud L, Lassalle JM, Frances B. Involvement of hippocampal CA3 K(ATP) channels in contextual memory.. Neuropharmacology, 2008.