Peter John Bradley
Professor, MIMG, University of California Los Angeles
Laboratory Address:
MSB 2550
Lab Number:
310-825-9087
Office Address:
2610B MSB
609 Charles E Young Dr East
Los Angeles, CA 90095
Office Phone Number:
310-825-8386
Affiliations
Research Interests
Biography
Dr. Bradley received his bachelor’s degree in Biology from the University of California, San Diego. At UCSD, he worked with Dr. Douglass Forbes studying the composition and function of the mammalian nuclear pore complex. He then received his PhD in Molecular Biology from UCLA in Dr. Patricia Johnson’s lab where he studied protein targeting to the hydrogenosome in the early-diverging amitochondrial eukaryote, Trichomonas vaginalis. In conjunction with phylogenetic analyses, his work on hydrogenosomal protein import was important for the conclusion that hydrogenosomes and mitochondria share a common origin.
Dr. Bradley continued his interest in parasites and their unique organelles as a postdoctoral fellow in Dr. John Boothroyd’s lab at Stanford University studying the opportunistic pathogen Toxoplasma gondii. His research focused on the role of the secretory rhoptries in host cell invasion and host-pathogen interactions. He carried out a proteomic analysis of the rhoptries that served as a foundation for many studies that elucidated how this organelle enables the parasite to infect is human host cell, hijack host functions, and cause disease.
Publications
A selected list of publications:
- Back Peter S, O’Shaughnessy William J, Moon Andy S, Dewangan Pravin S, Reese Michael L, Bradley Peter J Multivalent Interactions Drive the Toxoplasma AC9:AC10:ERK7 Complex To Concentrate ERK7 in the Apical Cap mBio, 2022; e0286421.
- Mineo Tiago W P, Chern Jessica H, Thind Amara C, Mota Caroline M, Nadipuram Santhosh M, Torres Juan A, Bradley Peter J Efficient Gene Knockout and Knockdown Systems in Neospora caninum Enable Rapid Discovery and Functional Assessment of Novel Proteins mSphere, 2022; e0089621.
- Chern Jessica H, Pasquarelli Rebecca R, Moon Andy S, Chen Allan L, Sha Jihui, Wohlschlegel James A, Bradley Peter J A Novel Toxoplasma Inner Membrane Complex Suture-Associated Protein Regulates Suture Protein Targeting and Colocalizes with Membrane Trafficking Machinery mBio, 2021; 12(5): e0245521.
- Torres Juan A, Pasquarelli Rebecca R, Back Peter S, Moon Andy S, Bradley Peter J Identification and Molecular Dissection of IMC32, a Conserved Toxoplasma Inner Membrane Complex Protein That Is Essential for Parasite Replication mBio, 2021; 12(1): .
- Nadipuram Santhosh Mukund, Thind Amara Cervantes, Rayatpisheh Shima, Wohlschlegel James Akira, Bradley Peter John Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites PloS One, 2020; 15(5): e0232552.
- Bradley Peter J, Rayatpisheh Shima, Wohlschlegel James A, Nadipuram Santhosh M Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii Methods in molecular biology (Clifton, N.J.), 2020; 2071: 323-346.
- Back Peter S, O’Shaughnessy William J, Moon Andy S, Dewangan Pravin S, Hu Xiaoyu, Sha Jihui, Wohlschlegel James A, Bradley Peter J, Reese Michael L Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis Proceedings of the National Academy of Sciences of the United States of America, 2020; .
- Choi Charles Paul, Moon Andy Seong, Back Peter Sungmin, Jami-Alahmadi Yasaman, Vashisht Ajay Amar, Wohlschlegel James Akira, Bradley Peter John A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex PLoS Biology, 2019; 17(10): e3000475.
- Suarez Catherine, Lentini Gaëlle, Ramaswamy Raghavendran, Maynadier Marjorie, Aquilini Eleonora, Berry-Sterkers Laurence, Cipriano Michael, Chen Allan L, Bradley Peter, Striepen Boris, Boulanger Martin J, Lebrun Maryse A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites Nature communications, 2019; 10(1): 4041.
- Chen Allan L, Moon Andy S, Bell Hannah N, Huang Amy S, Vashisht Ajay A, Toh Justin Y, Lin Andrew H, Nadipuram Santhosh M, Kim Elliot W, Choi Charles P, Wohlschlegel James A, Bradley Peter J Novel insights into the composition and function of the Toxoplasma IMC sutures Cellular Microbiology, 2017; .
- Wang Kevin, Peng Eric D, Huang Amy S, Xia Dong, Vermont Sarah J, Lentini Gaelle, Lebrun Maryse, Wastling Jonathan M, Bradley Peter J Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Vicia villosa Lectin Chromatography PloS one, 2016; 11(3): e0150561.
- Nadipuram Santhosh M, Kim Elliot W, Vashisht Ajay A, Lin Andrew H, Bell Hannah N, Coppens Isabelle, Wohlschlegel James A, Bradley Peter J In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis mBio, 2016; 7(4): .
- Mota Caroline M, Oliveira Ana C M, Davoli-Ferreira Marcela, Silva Murilo V, Santiago Fernanda M, Nadipuram Santhosh M, Vashisht Ajay A, Wohlschlegel James A, Bradley Peter J, Silva João S, Mineo José R, Mineo Tiago W P Neospora caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity Frontiers in microbiology, 2016; 7: 1456.
- Kim Elliot W, Nadipuram Santhosh M, Tetlow Ashley L, Barshop William D, Liu Philip T, Wohlschlegel James A, Bradley Peter J The Rhoptry Pseudokinase ROP54 Modulates Toxoplasma gondii Virulence and Host GBP2 Loading mSphere, 2016; 1(2): .
- Chen, AL Kim, EW Toh, JY Vashisht, AA Rashoff, AQ Van, C Huang, AS Moon, AS Bell, HN Bentolila, LA Wohlschlegel, JA Bradley, PJ Novel Components of the Toxoplasma Inner Membrane Complex Revealed by BioID MBio, 2015; 6(1): e02357-14.
- Gold DA Kaplan AD Lis A Bett GC Rosowski EE Cirelli KM Bougdour A Sidik SM Beck JR Lourido S Egea PF Bradley PJ Hakimi MA Rasmusson RL Saeij JP The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole Cell Host Microbe, 2015; 17(5): 642-52. .
- Silmon de Monerri Natalie C, Yakubu Rama R, Chen Allan L, Bradley Peter J, Nieves Edward, Weiss Louis M, Kim Kami The Ubiquitin Proteome of Toxoplasma gondii Reveals Roles for Protein Ubiquitination in Cell-Cycle Transitions Cell host & microbe, 2015; 18(5): 621-33.
- Rodriguez Jose A, Xu Rui, Chen Chien-Chun, Huang Zhifeng, Jiang Huaidong, Chen Allan L, Raines Kevin S, Pryor Alan, Nam Daewoong, Wiegart Lutz, Song Changyong, Madsen Anders, Chushkin Yuriy, Zontone Federico, Bradley Peter J, Miao Jianwei Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells IUCrJ, 2015; 2(Pt 5): 575-83.
- Beck JR Chen AL Kim EW Bradley PJ RON5 Is Critical for Organization and Function of the Toxoplasma Moving Junction Complex PLoS Pathogens, 2014; 10(3): e1004025.
- Tonkin ML Beck JR Bradley PJ Boulanger M The inner membrane complex sub-compartment proteins critical for replication of the apicomplexan parasite Toxoplasma gondii adopt a Pleckstrin homology fold, J Biol Chem, 2014; 289(20): 13962-73.
- de Leon JC, Scheumann N, Beatty W, Beck JR, Tran JQ, Yau C, Bradley PJ, Gull K, Wickstead B, Morrissette NS. A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components Eukaryot Cell, 2013; 12(7): 1009-19.
- Beck JR, Fung C, Straub KW, Coppens I, Vashisht AA, Wohlschlegel JA, Bradley PJ. A Toxoplasma palmitoyl acyl transferase and the palmitoylated Armadillo Repeat protein TgARO govern apical rhoptry tethering and reveal a critical role for the rhoptries in host cell invasion but not egress PLoS Pathog, 2013; 9(2): e1003162.
- Child MA, Hall CI, Beck JR, Ofori LO, Albrow VE, Garland M, Bowyer PW, Bradley PJ, Powers JC, Boothroyd JC, Weerapana E, Bogyo M. Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion Nat Chem Biol, 2013; 9(10): 651-6.
- Anderson-White B. Beck, J.R. Chen, C.T. Meissner M. Bradley P.J. Gubbels M.J. Cytoskeleton Assembly in Toxoplasma gondii Cell Division Int Rev Cell Mol Biol. , 2012; 298: 1-31.
- Hajagos, B.E. Turetzky, J.M. Peng, E.D. Cheng, S.J. Ryan, C.M. Souda, P. Whitelegge, J.P. Lebrun, M. Dubremetz, J.F. Bradley, P.J. Molecular Dissection of Novel Trafficking and Processing of the Toxoplasma gondii Rhoptry Metalloprotease Toxolysin-1 Traffic, 2012; 13(2): 292-304.
- Tonkin M.L. Brown S. Beck J.R. Bradley P.J. Boulanger M.J. Purification, crystallization and preliminary X-ray diffraction analysis of inner membrane complex (IMC) subcompartment protein 1 (ISP1) from Toxoplasma gondii. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012; 68(7): 832-4.
- Fung, C* Beck, J.R.* Robertson SD Gubbels MJ Bradley PJ Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation Mol Biochem Parasitol, 2012; 184(2): 99-108.
- Hajagos Bettina E, Turetzky Jay M, Peng Eric D, Cheng Stephen J, Ryan Christopher M, Souda Puneet, Whitelegge Julian P, Lebrun Maryse, Dubremetz Jean-Francois, Bradley Peter J Molecular dissection of novel trafficking and processing of the Toxoplasma gondii rhoptry metalloprotease toxolysin-1 Traffic (Copenhagen, Denmark), 2012; 13(2): 292-304.
- Sohn, C.S. Cheng, T.T. Drummond, M.L. Peng, E.D. Vermont, S.J. Xia, D. Cheng, S.J. Wastling J.M. Bradley, P.J. Identification of novel proteins in Neospora caninum using an organelle purification and monoclonal antibody approach PLoS One, 2011; 6(4): e18383.
Download - Liang, L., Döskaya, M., Juarez, S., Caner, A., Jasinskas, A., Tan, X., Hajagos, B.E., Bradley, P.J., Korkmaz, M., Güruz, Y., Felgner, P., Davies, D.H. Identification of potential serodiagnostic and subunit vaccine antigens by antibody profiling of toxoplasmosis cases in Turkey Mol Cell Proteomics, 2011; 10(7): M110.006916.
- Straub, K.W. Peng, E.D. Hajagos, B.E. Tyler, J.S. Bradley, P.J. The Moving Junction Protein RON8 Facilitates Firm Attachment and Host Cell Invasion in Toxoplasma gondii PLoS Pathogens, 2011; 7(3): e1002007.
Download - Beck, J.R., Rodriguez-Fernandez, I.A., de Leon, J.C., Huynh, M., Carruthers, V.B., Morrissette, N.S., Bradley, P.J. A Novel Family of Toxoplasma IMC Proteins Display a Hierarchical Organization and Function in Coordinating Parasite Division PLoS Pathogens, 2010; 6(9): pii: e1001094.
Download - Turetzky, J.M. Chu, D.K. Hajagos, B.E. Bradley, P.J. Processing and Secretion of ROP13: a unique Toxoplasma effector protein Int J Parasitol, 2010; 40(9): 1037-44.
- Breinich, M.S. Ferguson, D.J.P. Foth, B.J. van Dooren, G.G. Lebrun, M. Quon, D.V. Striepen, B. Bradley, P.J. Frischknecht, F. Carruthers, V.B. Meissner, M. A Dynamin is Required for the Biogenesis of Secretory Organelles in Toxoplasma gondii Current Biology, 2009; 19(4): 277-86.
- Straub, K.W. Cheng, S.J. Sohn, C.S. Bradley, P.J. Novel components of the Apicomplexan moving junction reveal conserved and coccidia-restricted elements Cellular Microbiology, 2008; 11(40): 590-603.
- Rome, M.E. Beck, J.R. Turetzky, J.M. Webster, P. Bradley, P.J. Intervacuolar transport and unique topology of GRA14, a novel dense granule protein in Toxoplasma gondii Infection and Immunity, 2008; 76(11): 4865-75. Epub 2008 Sep 2..
- Derocher AE, Coppens I, Karnataki A, Gilbert LA, Rome ME, Feagin JE, Bradley PJ, Parsons M. A thioredoxin family protein of the apicoplast periphery identifies abundant candidate transport vesicles in Toxoplasma Eukaryot Cell, 2008; 7: 1518-1529.
- Bradley, P.J. Sibley, L.D. Rhoptries: an arsenal of secreted virulence factors. Curr Opin Microbiol, 2007; 10(6): 582-7.
- Wastling, J.M. Bradley, P.J. Proteomic Analysis of the Rhoptry Organelles of Toxoplasma gondii, In “The biology of Toxoplasma gondii”, 2007; D. Soldati and J. Ajioka (eds.): 455-474.
- Gilbert L.A. Ravindran S. Turetzky J.M. Boothroyd J.C. Bradley P.J. Toxoplasma gondii Targets a Protein Phosphatase 2C to the Nucleus of Infected Host Cells Eukaryot Cell, 2007; 6(1): 73-83. Epub 2006 Nov 3. .
- Alexander, D.L., Mital, J., Ward, G.E., Bradley, P.J., Boothroyd, J.C. Identification of the Moving Junction Complex of Toxoplasma gondii: A Collaboration between Distinct Secretory Organelles PLOS Pathogens, 2005; 1(2): e17.
- Bradley, P.J., Ward, C., Cheng, S.J., Alexander, D.L., Coller, S., Coombs, G.H., Dunn, J.D., Ferguson, D.J., Sanderson, S.J., Wastling, J.M., Boothroyd, J.C. Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii The Journal of Biological Chemistry , 2005; 280(40): 34245-58.
- Lebrun M., Michelin A., Hajj H.E., Poncet J., Bradley P.J., Vial H., Dubremetz J.F. The rhoptry neck protein RON4 relocalizes at the moving junction during Toxoplasma gondii invasion Cellular Microbiology, 2005; 7(12): 1823-33.
- Bradley, P.J., Li, N., Boothroyd, J.C. A GFP-based motif-trap reveals a novel mechanism of targeting for the Toxoplasma ROP4 protein Molecular and Biochemical Parasitology. , 2004; 137(1): 111-20.
- Bradley, P.J., Hsieh, C.L., Boothroyd, J.C. Unprocessed Toxoplasma ROP1 is effectively targeted and secreted into the nascent parasitophorous vacuole Molecular and Biochemical Parasitology, 2002; 125(1-2): 189-93.
- Bradley, P.J., Boothroyd, J.C. The pro region of Toxoplasma ROP1 is a rhoptry-targeting signal International Journal for Parasitology. , 2001; 31(11): 1177-86.
- Hehl, A.B., Lekutis, C., Grigg, M.E., Bradley, P.J., Dubremetz, J.F., Ortega-Barria, E., Boothroyd, J.C. Toxoplasma gondii homologue of plasmodium apical membrane antigen 1 is involved in invasion of host cells Infection and Immunity. , 2000; 68(12): 7078-86.
- Dyall S D, Koehler C M, Delgadillo-Correa M G, Bradley P J, Plümper E, Leuenberger D, Turck C W, Johnson P J Presence of a member of the mitochondrial carrier family in hydrogenosomes: conservation of membrane-targeting pathways between hydrogenosomes and mitochondria Molecular and cellular biology, 2000; 20(7): 2488-97.
- Plumper E, Bradley P J, Johnson P J Competition and protease sensitivity assays provide evidence for the existence of a hydrogenosomal protein import machinery in Trichomonas vaginalis Molecular and biochemical parasitology, 2000; 106(1): 11-20.
- Bradley, P.J., Boothroyd, J.C. Identification of the pro-mature processing site of Toxoplasma ROP1 by mass spectrometry Molecular and Biochemical Parasitology, 1999; 100(1): 103-9.
- Plumper E, Bradley P J, Johnson P J Implications of protein import on the origin of hydrogenosomes Protist, 1998; 149(4): 303-11.
- Bradley P J, Lahti C J, Plümper E, Johnson P J Targeting and translocation of proteins into the hydrogenosome of the protist Trichomonas: similarities with mitochondrial protein import The EMBO journal, 1997; 16(12): 3484-93.
- Bui E T, Bradley P J, Johnson P J A common evolutionary origin for mitochondria and hydrogenosomes Proceedings of the National Academy of Sciences of the United States of America, 1996; 93(18): 9651-6.
- Lahti C J, Bradley P J, Johnson P J Molecular characterization of the alpha-subunit of Trichomonas vaginalis hydrogenosomal succinyl CoA synthetase Molecular and biochemical parasitology, 1994; 66(2): 309-18.
- Johnson P J, Lahti C J, Bradley P J Biogenesis of the hydrogenosome in the anaerobic protist Trichomonas vaginalis The Journal of parasitology, 1993; 79(5): 664-70.
- Finlay D R, Meier E, Bradley P, Horecka J, Forbes D J A complex of nuclear pore proteins required for pore function The Journal of cell biology, 1991; 114(1): 169-83.
Publications
- Back PS, Moon AS, Pasquarelli RR, Bell HN, Torres JA, Chen AL, Sha J, Vashisht AA, Wohlschlegel JA, Bradley PJ. IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome.. mBio, 2023.
- Oliveira Souza RO, Jacobs KN, Back PS, Bradley PJ, Arrizabalaga G. IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii.. Journal of cell science, 2022.
- Mineo TWP, Chern JH, Thind AC, Mota CM, Nadipuram SM, Torres JA, Bradley PJ. Efficient Gene Knockout and Knockdown Systems in Neospora caninum Enable Rapid Discovery and Functional Assessment of Novel Proteins.. mSphere, 2022.
- Chern JH, Pasquarelli RR, Moon AS, Chen AL, Sha J, Wohlschlegel JA, Bradley PJ. A Novel Toxoplasma Inner Membrane Complex Suture-Associated Protein Regulates Suture Protein Targeting and Colocalizes with Membrane Trafficking Machinery.. mBio, 2021.
- Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Reese ML, Bradley PJ. Multivalent Interactions Drive the Toxoplasma AC9:AC10:ERK7 Complex To Concentrate ERK7 in the Apical Cap.. mBio, 2021.
- Torres JA, Pasquarelli RR, Back PS, Moon AS, Bradley PJ. Identification and Molecular Dissection of IMC32, a Conserved Toxoplasma Inner Membrane Complex Protein That Is Essential for Parasite Replication.. mBio, 2021.
- Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Hu X, Sha J, Wohlschlegel JA, Bradley PJ, Reese ML. Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis.. Proceedings of the National Academy of Sciences of the United States of America, 2020.
- Nadipuram SM, Thind AC, Rayatpisheh S, Wohlschlegel JA, Bradley PJ. Proximity biotinylation reveals novel secreted dense granule proteins of Toxoplasma gondii bradyzoites.. PloS one, 2020.
- Bradley PJ, Rayatpisheh S, Wohlschlegel JA, Nadipuram SM. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii.. Methods in molecular biology (Clifton, N.J.), 2020.
- Choi CP, Moon AS, Back PS, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel JA, Bradley PJ. A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex.. PLoS biology, 2019.
- Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry-Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B, Boulanger MJ, Lebrun M. A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites.. Nature communications, 2019.
- Mota CM, Oliveira ACM, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TWP. Corrigendum: Neospora Caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity.. Frontiers in microbiology, 2019.
- Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. Retraction Note: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins.. Scientific reports, 2018.
- Mota CM, Chen AL, Wang K, Nadipuram S, Vashisht AA, Wohlschlegel JA, Mineo TWP, Bradley PJ. New molecular tools in Neospora caninum for studying apicomplexan parasite proteins.. Scientific reports, 2017.
- Chen AL, Moon AS, Bell HN, Huang AS, Vashisht AA, Toh JY, Lin AH, Nadipuram SM, Kim EW, Choi CP, Wohlschlegel JA, Bradley PJ. Novel insights into the composition and function of the Toxoplasma IMC sutures.. Cellular microbiology, 2016.
- Mota CM, Oliveira AC, Davoli-Ferreira M, Silva MV, Santiago FM, Nadipuram SM, Vashisht AA, Wohlschlegel JA, Bradley PJ, Silva JS, Mineo JR, Mineo TW. Neospora caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity.. Frontiers in microbiology, 2016.
- Nadipuram SM, Kim EW, Vashisht AA, Lin AH, Bell HN, Coppens I, Wohlschlegel JA, Bradley PJ. In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis.. mBio, 2016.
- Kim EW, Nadipuram SM, Tetlow AL, Barshop WD, Liu PT, Wohlschlegel JA, Bradley PJ. The Rhoptry Pseudokinase ROP54 Modulates Toxoplasma gondii Virulence and Host GBP2 Loading.. mSphere, 2016.
- Wang K, Peng ED, Huang AS, Xia D, Vermont SJ, Lentini G, Lebrun M, Wastling JM, Bradley PJ. Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Vicia villosa Lectin Chromatography.. PloS one, 2016.
- Silmon de Monerri NC, Yakubu RR, Chen AL, Bradley PJ, Nieves E, Weiss LM, Kim K. The Ubiquitin Proteome of Toxoplasma gondii Reveals Roles for Protein Ubiquitination in Cell-Cycle Transitions.. Cell host & microbe, 2015.
- Rodriguez JA, Xu R, Chen CC, Huang Z, Jiang H, Chen AL, Raines KS, Pryor A, Nam D, Wiegart L, Song C, Madsen A, Chushkin Y, Zontone F, Bradley PJ, Miao J. Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells.. IUCrJ, 2015.
- Gold DA, Kaplan AD, Lis A, Bett GC, Rosowski EE, Cirelli KM, Bougdour A, Sidik SM, Beck JR, Lourido S, Egea PF, Bradley PJ, Hakimi MA, Rasmusson RL, Saeij JP. The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole.. Cell host & microbe, 2015.
- Chen AL, Kim EW, Toh JY, Vashisht AA, Rashoff AQ, Van C, Huang AS, Moon AS, Bell HN, Bentolila LA, Wohlschlegel JA, Bradley PJ. Novel components of the Toxoplasma inner membrane complex revealed by BioID.. mBio, 2015.
- Tonkin ML, Beck JR, Bradley PJ, Boulanger MJ. The inner membrane complex sub-compartment proteins critical for replication of the apicomplexan parasite Toxoplasma gondii adopt a pleckstrin homology fold.. The Journal of biological chemistry, 2014.
- Beck JR, Chen AL, Kim EW, Bradley PJ. RON5 is critical for organization and function of the Toxoplasma moving junction complex.. PLoS pathogens, 2014.
- Child MA, Hall CI, Beck JR, Ofori LO, Albrow VE, Garland M, Bowyer PW, Bradley PJ, Powers JC, Boothroyd JC, Weerapana E, Bogyo M. Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion.. Nature chemical biology, 2013.
- de Leon JC, Scheumann N, Beatty W, Beck JR, Tran JQ, Yau C, Bradley PJ, Gull K, Wickstead B, Morrissette NS. A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components.. Eukaryotic cell, 2013.
- Beck JR, Fung C, Straub KW, Coppens I, Vashisht AA, Wohlschlegel JA, Bradley PJ. A Toxoplasma palmitoyl acyl transferase and the palmitoylated armadillo repeat protein TgARO govern apical rhoptry tethering and reveal a critical role for the rhoptries in host cell invasion but not egress.. PLoS pathogens, 2013.
- Tonkin ML, Brown S, Beck JR, Bradley PJ, Boulanger MJ. Purification, crystallization and preliminary X-ray diffraction analysis of inner membrane complex (IMC) subcompartment protein 1 (ISP1) from Toxoplasma gondii.. Acta crystallographica. Section F, Structural biology and crystallization communications, 2012.
- Fung C, Beck JR, Robertson SD, Gubbels MJ, Bradley PJ. Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation.. Molecular and biochemical parasitology, 2012.
- Anderson-White B, Beck JR, Chen CT, Meissner M, Bradley PJ, Gubbels MJ. Cytoskeleton assembly in Toxoplasma gondii cell division.. International review of cell and molecular biology, 2012.
- Hajagos BE, Turetzky JM, Peng ED, Cheng SJ, Ryan CM, Souda P, Whitelegge JP, Lebrun M, Dubremetz JF, Bradley PJ. Molecular dissection of novel trafficking and processing of the Toxoplasma gondii rhoptry metalloprotease toxolysin-1.. Traffic (Copenhagen, Denmark), 2011.
- Liang L, Döskaya M, Juarez S, Caner A, Jasinskas A, Tan X, Hajagos BE, Bradley PJ, Korkmaz M, Gürüz Y, Felgner PL, Davies DH. Identification of potential serodiagnostic and subunit vaccine antigens by antibody profiling of toxoplasmosis cases in Turkey.. Molecular & cellular proteomics : MCP, 2011.
- Sohn CS, Cheng TT, Drummond ML, Peng ED, Vermont SJ, Xia D, Cheng SJ, Wastling JM, Bradley PJ. Identification of novel proteins in Neospora caninum using an organelle purification and monoclonal antibody approach.. PloS one, 2011.
- Straub KW, Peng ED, Hajagos BE, Tyler JS, Bradley PJ. The moving junction protein RON8 facilitates firm attachment and host cell invasion in Toxoplasma gondii.. PLoS pathogens, 2011.
- Beck JR, Rodriguez-Fernandez IA, de Leon JC, Huynh MH, Carruthers VB, Morrissette NS, Bradley PJ. A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division.. PLoS pathogens, 2010.
- Turetzky JM, Chu DK, Hajagos BE, Bradley PJ. Processing and secretion of ROP13: A unique Toxoplasma effector protein.. International journal for parasitology, 2010.
- Breinich MS, Ferguson DJ, Foth BJ, van Dooren GG, Lebrun M, Quon DV, Striepen B, Bradley PJ, Frischknecht F, Carruthers VB, Meissner M. A dynamin is required for the biogenesis of secretory organelles in Toxoplasma gondii.. Current biology : CB, 2009.
- Straub KW, Cheng SJ, Sohn CS, Bradley PJ. Novel components of the Apicomplexan moving junction reveal conserved and coccidia-restricted elements.. Cellular microbiology, 2008.
- Rome ME, Beck JR, Turetzky JM, Webster P, Bradley PJ. Intervacuolar transport and unique topology of GRA14, a novel dense granule protein in Toxoplasma gondii.. Infection and immunity, 2008.
- DeRocher AE, Coppens I, Karnataki A, Gilbert LA, Rome ME, Feagin JE, Bradley PJ, Parsons M. A thioredoxin family protein of the apicoplast periphery identifies abundant candidate transport vesicles in Toxoplasma gondii.. Eukaryotic cell, 2008.
- Bradley PJ, Sibley LD. Rhoptries: an arsenal of secreted virulence factors.. Current opinion in microbiology, 2007.
- Gilbert LA, Ravindran S, Turetzky JM, Boothroyd JC, Bradley PJ. Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells.. Eukaryotic cell, 2006.
- Lebrun M, Michelin A, El Hajj H, Poncet J, Bradley PJ, Vial H, Dubremetz JF. The rhoptry neck protein RON4 re-localizes at the moving junction during Toxoplasma gondii invasion.. Cellular microbiology, 2005.
- Alexander DL, Mital J, Ward GE, Bradley P, Boothroyd JC. Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles.. PLoS pathogens, 2005.
- Bradley PJ, Ward C, Cheng SJ, Alexander DL, Coller S, Coombs GH, Dunn JD, Ferguson DJ, Sanderson SJ, Wastling JM, Boothroyd JC. Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii.. The Journal of biological chemistry, 2005.
- Bradley PJ, Li N, Boothroyd JC. A GFP-based motif-trap reveals a novel mechanism of targeting for the Toxoplasma ROP4 protein.. Molecular and biochemical parasitology, 2004.
- Bradley PJ, Hsieh CL, Boothroyd JC. Unprocessed Toxoplasma ROP1 is effectively targeted and secreted into the nascent parasitophorous vacuole.. Molecular and biochemical parasitology, 2002.